Chromatography

Background Chlorophyll often hides the other pigments present in leaves. In autumn, chlorophyll breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to read their colors. The liquify of pigments in a leaf may be illogical into bands of color by the technique of idea chromatography. Chromatography involves the breakup of kinds into somebody components. Chromatography means color writing. With this technique the components of a mixture in a liquid medium argon spaced. The legal separation takes orchestrate by assiduity and capillarity. The paper holds the substances by absorption; capillarity pulls the substances up the paper at different rates. Pigments atomic number 18 separated on the paper and show up as dark streaks. The pattern of separated components on the paper is called a chromatogram. Question Which pigments underside you identify in a third estate leaf? Prediction The pigments that ask on on the chromatograph paper should be rela ted to pigments seen on plants in real life. go forth of various possibilities, I believe that trine pigments that may be disc overed in this lab allow be carotene, chlorophyll a, and xanthrophylls. Safety Precautions The solvent is volatile. Ensure that in that respect are no flames in the schoolroom, and avoid breathing vapors from the solvent.

The classroom must be well-ventilated Materials * Spinach leaves * Solvent (90% rock embrocate ether and 10% acetone) * Chromatography paper * Paper clip * heel counter stand * Test-tube clamp * Cork stopper * come aft( prenominal) glass * Large evidence tub! e Procedure 1. conquer the large test tube to a retort stand. 2. repose up the bobsleigh stopper and the paper clip as shown. 3. Measure a piece of chromatography paper so that it is languish enough to go down from the paper clip precisely not so long that it touches the bottom of the test tube. (Refer to the diagrams). burn down the paper to a advert at one end. 4. fleck a coleus or spinach leaf over the pointed end of the chromatography...If you want to get a full essay, recount it on our website: BestEssayCheap.com

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